^{Laboratory for Cellular Metabolism and Engineering} _{LCME Analytics}

Part of the Murdock Metabolomics Laboratory

Overseen by the Gang Lab

Focus areas:

Mass spectrometry-based tissue imaging (imaging mass spectrometry) on a Waters Synapt G2 HDMS system, focusing primarily on plant tissues, although animal or other samples (e.g., microbial mats or colonies) are analyzed as well
Primary metabolite analysis using GC-TOFMS (LECO Pegasus IV GC(xGC)-TOFMS), where several hundred primary metabolites (amino acids, sugars, organic acids, fatty acids, volatiles, etc.) are routinely identified and quantified
Targeted metabolite analysis using LC-MS/MS (Waters Xevo TQ triple quad), where specific methods are developed for high sensitivity metabolite analysis
- An example of this area is our establishment of standard methods for cannabinoid quantification from biological samples
  - Delta-9-tetrahydrocannabinol (THC), cannabidiol (CBD) and over a dozen plus other (phyto)cannabinoids
  - Endocannabinoids (the natural compounds produced in the human brain that naturally bind to the cannabinoid receptor)
- Another example is the quantification of L-dopa and dopamine, both in plant and animal samples
- An additional example is the quantification of specific flavonoids, such as mint family trichome flavones
- A fourth example is the quantification of curcumin and related curcuminoids, diarylheptanoids and gingerols
Non-targeted LC-MS/MS-based metabolomic analysis using a LC-MS/MS system (Waters Synapt G2-S HDMS) and multivariate statistical analyses
Non-targeted and targeted lipidomic analysis on the Waters Synapt G2-S, including membrane lipid profiling

LCME Analytics is a University Service Center, providing the WSU and greater Pacific Northwest Research communities with metabolomics-based support.